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Colorimetry Guide

3/2/2015 7:31:00 AM by Labequipmentdepot: Authorized Lab Equipment Distributor

| Many biochemical experiments involve the measurements of compound or group of compounds present in a complex mixture. The most widely used method for determining the concentration of biochemical compounds is colorimetry, which makes use of the property that when white light passes through a colored solution, some wavelength are absorbed more than others. Many compounds are not themselves colored but can be made to absorb light in visible region by reaction with suitable reagents. These reactions are fairly specific and in most cases very sensitive, so that quantities of material in the region of mol / L concentrations can be measured. The big advantage of is that complete isolation of compound is not necessary and the constituents of a complex mixture such as blood can be determined after little treatment. The depth of the color is proportional to the concentration of the compound being measured, while the amount of light is proportional to the intensity of the color and hence the concentration.


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